Technical Data
S0099-02
Scavenger Receptor BI (SR-BI)
Description:
SR-BI is a high density lipoprotein (HDL) receptor. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidogenic tissues, and mediates selective cholesterol uptake by a mechanism distinct from the classic LDL receptor pathway (1). SR-BI mediates the selective uptake of cholesteryl esters from HDL and cholesterol secretion into bile in the liver. CLAMP (C-terminal linking and modulating protein), a four-PDZ-domain-containing protein, is associated with SR-BI in the liver sinusoidal plasma membranes and may modulate the intracellular transport and metabolism of cholesteryl esters taken up from HDL (2). It has also been shown that the hepatitis C virus (HCV) envelope glycoprotein E2 selectively binds to human hepatic cells via SR-BI (3).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Immunohistochemistry: 1:100 -1:250
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E537Supernatant
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues near the N-terminus of human SR-B1.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human SR-B1 Scavenger Receptor at ~80kD. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Acton S, et al. Science 271:518-520 (1996). 2. Ikemoto M, et al. Proc Natl Acad Sci 97:6538-6543 (2000). 3. Scarselli E, et al. EMBO J, 21:5017-5025 (2002).