Technical Data
S0099-02
Scavenger Receptor BI (SR-BI)
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Technical Data |
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S0099-02 |
Scavenger Receptor BI (SR-BI) |
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Description: SR-BI is a high density lipoprotein (HDL) receptor. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidogenic tissues, and mediates selective cholesterol uptake by a mechanism distinct from the classic LDL receptor pathway (1). SR-BI mediates the selective uptake of cholesteryl esters from HDL and cholesterol secretion into bile in the liver. CLAMP (C-terminal linking and modulating protein), a four-PDZ-domain-containing protein, is associated with SR-BI in the liver sinusoidal plasma membranes and may modulate the intracellular transport and metabolism of cholesteryl esters taken up from HDL (2). It has also been shown that the hepatitis C virus (HCV) envelope glycoprotein E2 selectively binds to human hepatic cells via SR-BI (3). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Immunohistochemistry: 1:100 -1:250 Immunocytochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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