Technical Data
S0379-80C
Securin, NT (PTTG1, Pituitary Tumor-transforming Gene 1 Protein, hPTTG, Tumor-transforming Protein 1, Esp1-associated Protein, TUTR1, PTTG, EAP1)
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Technical Data |
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S0379-80C |
Securin, NT (PTTG1, Pituitary Tumor-transforming Gene 1 Protein, hPTTG, Tumor-transforming Protein 1, Esp1-associated Protein, TUTR1, PTTG, EAP1) |
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Description: PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The protein has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. This protein contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the protein can act as a transactivation domain. It is mainly a cytosolic protein, although it partially localizes in the nucleus. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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