Technical Data
SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor, SF)
Molecular Biology Storage: -20CShipping: Blue Ice
Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
SCF is a growth factor important for the survival, proliferation and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

Amino Acid Sequence:
The sequence of the first five amino acids was determined to be Met-Glu-Gly-Ile-Cys

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is <2ng/ml, corresponding to a Specific Activity of 5x10e5IU/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human recombinant SCF, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 165 amino acids.
Purity: Purified by chromatography.
Concentration: ~0.1mg/ml (after reconstitution)
Form: Supplied as a lyophilized powder from 10mM acetic acid. Reconstitute with 20ul sterile ddH2O. For long term storage, add a carrier protein (0.1% HSA or BSA).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.