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Technical Data |
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S0400-07S |
SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor, SF) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Recombinant Human Stem Cell Factor is a single, non-glycosylated, polypeptide chain containing 165aa and having a molecular weight of 18.4kD. The sequence of the first five N-terminal amino acids was determined to be Met-Glu-Gly-Ile-Cys. Contains less than 1% dimers and aggregates. Source: E. coli Molecular Weight: ~18.4kD Biological Activity: The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is <2ng/ml, corresponding to a specific activity of 2x10e5 IU/mg. Endotoxin: 0.1ng/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 18.4kD |
Source: E. coli Purity: 98% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE). Form: Supplied as a lyophilized powder from 10mM acetic acid. Reconstitute with sterile deionized water to a concentration 100ug/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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