Technical Data
S0400-09
SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, FPH2, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, SHEP7, Steel Factor, SF)
2ug
5ug
5ug
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Technical Data |
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S0400-09 |
SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, FPH2, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, SHEP7, Steel Factor, SF) |
2ug 5ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain. Source: DNA sequence encoding the signal peptide and the mature Stem Cell Factor (SCF) protein of human SCF was expressed in modified human 293 cells. Biological. Activity: The ED50 of SCF is typically 10-20 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent M-07e cell line. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: SCF migrates as a broad band between ~20 and 42kD in SDS-PAGE due to post-translational modifications. Unmodified SCF has a predicted molecular mass of 21.0kD. SCF separates into a number of isoforms with a pI between 3.0 and 7.0 in 2D PAGE due to post-translational modifications. This compares with the unmodified SCF with a predicted pI of 5.76. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: Modified human 293 cells Purity: ~95% (SDS-PAGE, silver stain) Concentration: As reported Form: Supplied as lyophilized powder from PBS, 1% HSA, 10% trehalose. Reconstitute with 500ul sterile PBS. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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