Technical Data
SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, FPH2, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, SHEP7, Steel Factor, SF)
Molecular Biology Storage: -20CShipping: Blue Ice
Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain.

DNA sequence encoding the signal peptide and the mature Stem Cell Factor (SCF) protein of human SCF was expressed in modified human 293 cells.

Biological. Activity:
The ED50 of SCF is typically 10-20 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent M-07e cell line.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: SCF migrates as a broad band between ~20 and 42kD in SDS-PAGE due to post-translational modifications. Unmodified SCF has a predicted molecular mass of 21.0kD. SCF separates into a number of isoforms with a pI between 3.0 and 7.0 in 2D PAGE due to post-translational modifications. This compares with the unmodified SCF with a predicted pI of 5.76.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Modified human 293 cells
Purity: ~95% (SDS-PAGE, silver stain)
Concentration: As reported
Form: Supplied as lyophilized powder from PBS, 1% HSA, 10% trehalose. Reconstitute with 500ul sterile PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.