Technical Data
Molecular Biology Storage: -20CShipping: Blue Ice
5'-A G T^A C T-3'
3'-T C A^T G A-5'

Concentration: 10u/ul
Source: Streptomyces caespitosus

Supplied with:
R1625-70: Restriction Enzyme Buffer for ScaI, SdaI, 10X
37mM Tris-acetate (pH 7.0), 15mM magnesium acetate, 150mM potassium acetate and 0.1mg/ml BSA. Incubate at 37C

Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periodsthe Storage Buffer should be used.

Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 5-fold overdigestion (5u/ug lambda DNA x 1 hour) with ScaI (see Star Activity).

Ligation/Recutting Assay: After 5-fold overdigestion (5u/ug DNA x 1 hour) with ScaI, more than 80% of the DNA fragments can be ligated at a 5'-termini concentration of 0.05uM. More than 90% of these can be recut.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.

Star Activity: A large excess of enzyme (7.5u/ug DNA x 1 hour) may result in star activity.

Stability during Prolonged Incubation: A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.

Thermal Inactivation: Only small amounts of enzyme (up to 10 units) can be inactivated by incubation at 80C for 20min.

Digestion of Agarose-embedded DNA: A minimum of 20 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Number of Recognition Sites in DNA:

Lambda: 5
PhiX174: 0
M13mp18/19: 0
pBR322: 1
pUC18/19: 1
pUC57: 1
pTZ19R/U: 1
pBluescriptIIKS(-/+): 1
pBluescriptIISK(-/+): 1
pACYC177: 1
pACYC184: 1
5 0 0 1 1 1 1 1 1 1 1

* Unit Definition. One unit is defined as the amount of enzyme required to digest 1g of lambda DNA in 1 hour at 37C in 50l of assay buffer.

NR: buffer is not recommended, since star activity appears at 5-fold overdigestion (5 units x 1 hour).

Product Use Limitation


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Updated March 07, 2002 15:46

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.