Technical Data
Scl-70 (Scleroderma, DNA Topoisomerase I)
Topoisomerase I changes the topology of DNA via cleavage of single strand only. Suitable for use in DNA conformational analysis and topology as well as DNA repair, drug resistance, cell proliferation and leukemia studies. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated if enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical segment is bent around and joined in a circle, the two strands are topologically linked, or knotted. They cannot be separated by any process that does not involve the breaking of strands. Topoisomerases catalyze and guide the unknotting of DNA.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Immunodiffusion: 1:8

Anti-La/SS-B Activity by ELISA: As Reported (lot-specific).

In an ELISA system designed to detect all IgG subclasses, the following purified antigens (dilution of 1:100) responded as:
Antigen Result (EU/ml)
SSA 15
SCL 102
Sm 3
Sm/RNP 2
Jo-1 8

Interpretation of Results:
<16 EU/ml: Negative
16-20 EU/ml: Equivocal
>20 EU/ml: Positive

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
2ml-20CBlue IceHumanHuman
Not Determined
Sci-70. Autoantibody derived from donor human serum.
Purified by double immunodiffusion against rabbit thymus extract.
Supplied as a lyophilized powder from PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Undiluted antibody shows a strong Scl-70 precipitin band. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. This product shows no reactivity at a dilution of 1:100 when tested against purified Sm/RNP, SS-A, SS-B, Sm or Jo-1 antigens in an ELISA system designed to detect all IgG subclasses. Exhibits strong positive in an Anti-Scl-70 ELISA system.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.