Technical Data
S0500-03
Scl-70 (Scleroderma, DNA Topoisomerase I)
Description:
Scl-70 (Topoisomerase I) changes the topology of DNA via cleavage of single strand only. Suitable for use in DNA conformational analysis and topology as well as DNA repair, drug resistance, cell proliferation and leukemia studies. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated if enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical segment is bent around and joined in a circle, the two strands are topologically linked, or knotted. They cannot be separated by any process that does not involve the breaking of strands. Topoisomerases catalyze and guide the unknotting of DNA.

Applications:
Suitable for use in ELISA and Immunodiffusion. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
2ml-20CBlue IceHumanHuman
Concentration:
Not determined.
Immunogen:
Human Scl-70 from plasma.
Purity:
Serum
Form
Supplied as a lyophilized powder from 0.01M PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Specificity:
Recognizes human Scl-70. Does not recognize purified SSA, SSB, Sm, Sm/RNp ot Jo-1 antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.