Technical Data
S0549
SdaI (Sse8387I)
300U
1500U
Molecular Biology Storage: -20CShipping: Blue Ice
5'-C C T G C A^G G-3'
3'-G G^A C G T C C-5'

Concentration:
~10u/ul

Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA fragments in 1 hour at 37C in 50ul of assay buffer.

Source:
E. coli that carries the cloned sdaIR gene from Streptomyces diastaticus Ng 7-324

Supplied With:
R1625: Restriction Enzyme Buffer A, 10X
R1625-70: Restriction Enzyme Buffer for ScaI, SdaI, 10X

Diluent Buffer:
For short term storage (~3-4 weeks)-10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periodsthe Storage Buffer should be used.

Storage Buffer:
10mM Tris-HCl (pH 7.5 at 25C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control:
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 5-fold overdigestion (5 units/ug lambda DNA x 1 hour) with SdaI (see Star Activity).

Ligation/Recutting Assay: After 5-fold overdigestion (2.5 units/ug DNA x 2 hours) with SdaI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.05uM. More than 95% of these can be recut.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.

Blue/White Cloning Assay: pUC19 DNA was digested at a unique site with 10 units of enzyme for 16 hours. After religation and transformation 0.1% of white colonies were detected.

Enzyme Properties:
Star Activity: An excess of enzyme
(7.5u/ug DNA x 1 hour) may result in star activity.

Methylation Effects:
Dam/Dcm/CpG/EcoKl/EcoBl: never overlaps - no effect.

Stability During Prolonged Incubation: A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.

Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min.

Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: Alw21I, Mph1103I, PstI, SduI

Number of Recognition Sites in DNA:
Lambda: 5
PhiX174: 0
M13mp18/19: 1
pBR322: 0
pUC18/19: 1
pUC57: 0
pTZ19R/U: 1

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.