Technical Data
S0630
Secretory Component
Description:
Secretory Component is an epithelial transport receptor for polymeric IgA and IgM. Antibody to secretory component has been shown to be useful for identifying glandular carcinomas.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:100-1:200 for 20-60min, RT. To stain formalin-fixed tissues, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20min, then cool 20min RT.
Optimal dilution determined by the researcher.

Positive Control:
Small intestine, stomach, colon carcinoma

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.556Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Semi-purified human secretory component (SC). Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes a single band of 80kD human glycoprotein, identified as secretory component. Reacts with both free and polymeric Ig-bound secretory component. Shows no reaction in Western Blots of cell lines lacking secretory component. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bartel, J., Tlaskalova-Hogenova, H., Staskova, Z., Simeckova, J., Vojtesek, B., Rejthar, A., Kovarik, J., Bartkova, J.: Secretory component in differentiating normal epithelium, benign lesions and malignancy in the human breast as monitored by monoclonal antibodies. Histochemistry 91: 235-244 (1989). 2. Kvale, D., Bartek, J., Sollid, L.M., Brandtzaeg, P.: Rapid selection of cultured cells with increased expression of a membrane marker (secretory component). Int. J. Cancer 42: 638-641 (1988).