Technical Data
Secretory Component
Secretory Component is an epithelial transport receptor for polymeric IgA and IgM. Antibody to secretory component has been shown to be useful for identifying glandular carcinomas.

Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:100-1:200 for 20 minutes at RT. To stain formalin-fixed tissues, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, then cooling at RT for 20 minutes.
Optimal dilution determined by the researcher.

Positive Control:
Small intestine, stomach, colon carcinoma


Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG10.N.556Affinity Purified
500ul-20CBlue IceHumanMouse
Semi-purified human secretory component (SC). Cellular Localization: Cytoplasmic.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Recognizes a single band of 80kD human glycoprotein, identified as secretory component. Reacts with both free and polymeric Ig-bound secretory component. Shows no reaction in Western Blots of cell lines lacking secretory component. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bartek, J., Tlaskalova-Hogenova, H., Staskova, Z., Simeckova, J., Vojtesek, B., Rejthar, A., Kovarik, J., Bartkova, J.: Secretory component in differentiating normal epithelium, benign lesions and malignancy in the human breast as monitored by monoclonal antibodies. Histochemistry 91: 235-244 (1989). 2. Kvale, D., Bartek, J., Sollid, L.M., Brandtzaeg, P.: Rapid selection of cultured cells with increased expression of a membrane marker (secretory component). Int. J. Cancer 42: 638-641 (1988).