		Technical Data
S0647-10	SEL1L (Suppressor of lin-12-like Protein)

Description:
SEL1L (suppressor of lin-12-like protein) is the human ortholog of the C. elegans sel-1 gene, a negative regulator of the lin-12/Notch family of proteins. Human SEL1L is a component of endoplasmic reticulum-associated degradation (ERAD), involved in ubiquitin-dependent degradation of misfolded ER proteins. In addition, SEL1L plays a role in cancer progression. Several studies provided significant evidence that increased SEL1L expression is linked to a decrease in tumor aggressiveness.

Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen and paraffin sections.
Western Blot: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6D756	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant human SEL1L.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 150mM sodium chloride, pH 7.4, 0.02% sodium azide.

Specificity:

Recognizes human SEL1L isoforms < 88kD. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Orlandi, R., et al., J. Biol. Markers 17: 104 (2002). 2. Orlandi, R., et al., Cancer Res. 62: 567 (2002).