Technical Data
S0647-10
SEL1L (Suppressor of lin-12-like Protein)
Description:
SEL1L (suppressor of lin-12-like protein) is the human ortholog of the C. elegans sel-1 gene, a negative regulator of the lin-12/Notch family of proteins. Human SEL1L is a component of endoplasmic reticulum-associated degradation (ERAD), involved in ubiquitin-dependent degradation of misfolded ER proteins. In addition, SEL1L plays a role in cancer progression. Several studies provided significant evidence that increased SEL1L expression is linked to a decrease in tumor aggressiveness.

Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen and paraffin sections.
Western Blot: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D756Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human SEL1L.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 150mM sodium chloride, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes human SEL1L isoforms < 88kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Orlandi, R., et al., J. Biol. Markers 17: 104 (2002). 2. Orlandi, R., et al., Cancer Res. 62: 567 (2002).