Technical Data
S0950-11
SEPT2 (Septin 2, DIFF6, KIAA0158, NEDD5, Pnutl3, hNedd5, neural precursor cell expressed, developmentally down-regulated 5)
Description:
This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. This gene encodes a protein associated with the tau-based paired helical filament core, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. SEPT2, otherwise known as septin-2, is a cytoskeletal GTPase and member of the septin family, essential for the formation of filaments, in conjunction with SEPT6 and SEPT7. SEPT2 plays an important role during mitosis progression/cytokinesis, in the correct organization of the actin cytoskeleton, and maintains polyGlu microtubule tracks, thereby facilitating epithelial cell vesicle transport.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000.
Western Blot: 1-3ug/ml. A band is detected at 40kD band observed in Human, Mouse and Rat Testis lysates (calculated MW of 41.5kD according to NP_001008491.1). Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See S0950-11-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
A synthetic peptide from the N-terminus of aa-SKQQPTQFINPET.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human SEPT2. Species sequence homology: mouse, rat and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclear
accumulation of NCK mediated by SOCS7. Cell. 2007 Sep 7;130(5):837-50.