Technical Data
S0950-12
SEPT7 (Septin 7, CDC10, CDC3, Nbla02942, SEPT7A, CDC10)
Description:
SEPT7, otherwise known as septin-7, is a cytoskeletal GTPase and member of the septin family, essential for the formation of filaments, in conjunction with SEPT2 and SEPT6. SEPT7 plays an important role during mitosis progression/cytokinesis, in the correct organization of the actin cytoskeleton, and in the association of centrosome-associated protein E (CENPE) with kinetochore.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000.
Western Blot: 0.3-1ug/ml. A band is detected at 45kD in nuclear lysates of cell line HeLa (calculated MW of 50.7kD according to NP_001779.3 and of 55.6kDa according to NP_001011553.2). Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See S0950-12-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
A synthetic peptide from the C-terminus of aa-DNNKNKGQLTKSP.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human SEPT7. Species sequence homology: mouse, rat, equine, canine and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclear
accumulation of NCK mediated by SOCS7. Cell. 2007 Sep 7;130(5):837-50.