Technical Data
S0976-40C
SERCA1 (L24) (Sarcoplasmic/endoplasmic Reticulum Calcium ATPase 1, SERCA 1 ATPase, SR Ca(2+) ATPase 1, ATPase Ca++ Transporting Cardiac Muscle Fast Twitch 1, ATPase Ca++ Transporting Fast Twitch 1, ATP2A, ATP2A1, Calcium Pump 1, Calcium Transporting ATPas
Description:
Sarcoplasmic and endoplasmic reticulum Ca2+ ATPases (SERCA) are members of a highly conserved family of Ca2+ pumps (1). SERCA pumps transport Ca2+ from the cytosol to the sarcoplasmic and endoplasmic reticulum lumen against a large concentration gradient (1). ATP2A1 (SERCA1) is a fast-twitch, skeletal muscle sarcoplasmic reticulum Ca2+ ATPase (2). Mutations in the correspond-ing gene cause an autosomal recessive muscle disorder known as Brody myopathy, which is characterized by muscle cramping and impaired muscle relaxation associated with exercise (1-3).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide derived from the sequence around Leu24 of human ATP2A1/SERCA1(KLH-coupled).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150 mM NaCl, 100 g/ml BSA and 50% glycerol
Specificity:
Recognizes human ATP2A1/SERCA1 (L24). Species crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hovnanian, A. (2007) Subcell Biochem 45, 337-63, 2. Odermatt, A. et al. (1996) Nat Genet 14, 191-4, 3. Odermatt, A. et al. (2000) Hum Genet 106, 482-91.