		Technical Data
S0976-40C	SERCA1 (L24) (Sarcoplasmic/endoplasmic Reticulum Calcium ATPase 1, SERCA 1 ATPase, SR Ca(2+) ATPase 1, ATPase Ca++ Transporting Cardiac Muscle Fast Twitch 1, ATPase Ca++ Transporting Fast Twitch 1, ATP2A, ATP2A1, Calcium Pump 1, Calcium Transporting ATPas

Description:
Sarcoplasmic and endoplasmic reticulum Ca2+ ATPases (SERCA) are members of a highly conserved family of Ca2+ pumps (1). SERCA pumps transport Ca2+ from the cytosol to the sarcoplasmic and endoplasmic reticulum lumen against a large concentration gradient (1). ATP2A1 (SERCA1) is a fast-twitch, skeletal muscle sarcoplasmic reticulum Ca2+ ATPase (2). Mutations in the correspond-ing gene cause an autosomal recessive muscle disorder known as Brody myopathy, which is characterized by muscle cramping and impaired muscle relaxation associated with exercise (1-3).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

A synthetic peptide derived from the sequence around Leu24 of human ATP2A1/SERCA1(KLH-coupled).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150 mM NaCl, 100 µg/ml BSA and 50% glycerol

Specificity:

Recognizes human ATP2A1/SERCA1 (L24). Species crossreactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hovnanian, A. (2007) Subcell Biochem 45, 337-63, 2. Odermatt, A. et al. (1996) Nat Genet 14, 191-4, 3. Odermatt, A. et al. (2000) Hum Genet 106, 482-91.