Technical Data
Serotonin Receptor 1D (5-Hydroxytryptamine (Serotonin) Receptor 1D, 5-Hydroxytryptamine Receptor 1D, 5HT1D, 5-HT1D, 5-HT-1D, Htr1d)
Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1,2). The 5-HT1D (formerly known as 5-HT1Da) receptor has 63% overall structural homology with the 5-HT1B receptor and a 77% aa sequence homology in the seven transmembrane domains (3). In situ hybridization studies have detected highest level of mRNA in primary olfactory cortex, accumbens nucleus, caudate putamen, lateral mammilary nucleus and medial vestibular nucleus (4). The presence of relatively high levels of 5-HT1D mRNA in the locus coeruleus suggest that, when expressed on nerve terminals, this receptor could modulate the release of catecholamines. The 5-HT1D receptor may function as an autoreceptor. Like 5-HT1B receptors, 5-HT1D receptors also appear to act as heteroreceptors. Acting on the 5-HT1D receptors, 5-HT appears to inhibit release of glutamate from rat cerebellar synaptosomes and acetylcholine from guinea pig hippocampal synaptosomes (5). It has been suggested that neurogenic inflammation and nociceptive activity within trigemino- vascular afferents. Endothelium-dependent relaxation in the pig coronary artery has been claimed to be mediated by 5-HT1D receptors (6). Like other members of the 5-HT1 family, 5-HT1D receptors are negatively coupled to adenylate cyclase.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human, mouse, or rat brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceRatRabbit
Synthetic peptides corresponding to aa1-18 and 251-267 of rat 5-HT1DR (Genbank accession no. NP_036984) (7).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.02% sodium azide.
Recognizes rat Serotonin Receptor 5-HT1D (5-HT1DR). Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Petrouka, SJ. Trends Neurosci. 11: 486-500 (1998). 2. Lauder, JM. Trends Neurosci. 16: 233-240 (1993). 3. Weinshank, RL., Zgombick, JM., Macchi, MJ., Branchek, TA., Hartig, PR. Proc. Acad. Natl. Sci. USA 89: 3630-3633 (1992). 4. Gerhardt, CC. And van Heerikhuizen, H. Eur. J. Pharmacol. 334: 1-23 (1997). 5. Leonhardt, S., Herrick-Davis, K., Titeler, M. Proc. Natl. Acad. Sci. USA 89: 5517-5521 (1989). 6. Miller, KJ. And Teitker, M. Neurosci. Lett. 136: 223-226 (1992). 7. Hamblin,M.W., McGuffin,R.W., Metcalf,M.A., Dorsa, et al. Mol. Cell. Neurosci. 3, 578-587 (1992).