		Technical Data
S1001-18L	Serotonin Receptor 2C (HTR2C, 5-hydroxytryptamine Receptor 2C, 5-HT-2C, 5-HT2C, 5-HTR2C, 5-hydroxytryptamine Receptor 1C, 5-HT-1C, 5-HT1C, HTR1C)

Description:
Serotonin Receptor 2C (5-HT2C) activates phospholipase C, resulting in a transient increase in intracellular calcium and activation of protein kinase C. Mutant mice lacking functional 5-HT2C receptor are overweight due to abnormal feeding behavior and are prone to epileptic seizures. Therefore, agonists of the 5-HT2C receptor such as m-chlorophenylpiperazine (mCPP) may be potential drugs for the treatment of obesity. However, mCPP also has unpleasant side effects such as anxiety and nausea in humans. Most psychedelic drugs bind 5-HT2A and 5-HT2C receptors, and their hallucinogenicity correlates with binding affinity. Expression of the 5-HT2C receptor has been reported predominantly in brain, but also in spinal cord and lung. ESTs have been isolated from human, mouse, and rat brain libraries.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 1-3ug/ml, observed in lysates of human EBV immortalised Lymphoblastoids, T cell MOLT4 and monocytic cell line U937 on ~50kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-QVENLELPVN, from human HTR2C, at the internal region of the protein (NP_000859.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human HTR2C. Species sequence homology: Bovine, canine, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Castensson A, Aberg K, McCarthy S, Saetre P, Andersson B, Jazin E. Serotonin receptor 2C (HTR2C) and schizophrenia: Examination of possible medication and genetic influences on expression levels. Am J Med Genet B Neuropsychiatr Genet. 2005 Feb 16.