Technical Data
S1001-19B
Serotonin Receptor 1B (5-Hydroxytryptamine (Serotonin) Receptor 1B, 5-Hydroxytryptamine Receptor 1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1B Receptor, 5-HT1DB, HTR1B, HTR1D2, HTR1DB, S12, Serotonin 1D beta Receptor)
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Technical Data |
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S1001-19B |
Serotonin Receptor 1B (5-Hydroxytryptamine (Serotonin) Receptor 1B, 5-Hydroxytryptamine Receptor 1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1B Receptor, 5-HT1DB, HTR1B, HTR1D2, HTR1DB, S12, Serotonin 1D beta Receptor) |
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Description: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1). Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase (2). Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum (3,4). The in situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: human, mouse, or rat brain Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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