Technical Data
S1001-19B1
Serotonin Receptor 1B (5-Hydroxytryptamine (Serotonin) Receptor 1B, 5-Hydroxytryptamine Receptor 1B, 5ht1b, 5-HT1B, 5-HT-1B, HTR1B)
Description:
Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1). Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase (2). Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum (3,4). The in situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei (5).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human, mouse, or rat brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptides corresponding to aa8-26 and 263-278 of rat 5-HT1BR (Accession number P28564) (6).
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes rat 5-HT1BR. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.