Technical Data
S1001-19K
Serotonin Receptor 3A (5-HT3A, 5-HT3-A, HTR3A, 5-Hydroxytryptamine (Serotonin) Receptor 3A, 5-Hydroxytryptamine Receptor 3, 5HT3, 5-HT-3, 5-HT3R, HTR3, Serotonin-gated Ion Channel Receptor)
Description:
Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1). The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems (2,3). Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455aa (4). The human subunit exhibits 85% and 84% aa sequence identity to the mouse (5) and rat (6) receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord (7).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptides corresponding to aa21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949.
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes rat 5-HT3AR. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Petrouka, SJ. Trends Neurosci. 11: 486-500 (1998). 2. Derkach, V., Surprenant, A., North, RA. Nature 339: 706-709 (1989). 3. Maricq, AV., Peterson, AS., Brake, AJ., Myers, RM., Julius, D. Science 254: 432-437 (1991). 4. Belleli, D., Balcarek, JM., Hope, AG., Peters, JA., Lambert, JJ., Blackburn, TP. Mol. Phrmacol. 48: 1054-1062 (1992). 5. Hope, AG., Downie, DL., Sutherland, L., Lambert, JJ., Peters, JA, Burchell, B. Eur. J. Pharmacol. 245: 87-192 (1993). 6. Johnson, DS. And Heinemann, SF. Soc. Neurosci. (abstract) 18: 249 (1992). 7. Morales, M., Battenberg, E., de Lecca, L., Sanna, PP., Bloom, F. Mol. Brain Res. 36: 251-260 (1996).