Technical Data
S1001-19L
Serotonin Receptor 3A (5-HT3A, HTR3A, 5-Hydroxytryptamine (Serotonin) Receptor 3A, 5-HT-3, 5-HT3R, HTR3, 5HT3 Serotonin Receptor, Serotonin-gated Ion Channel Receptor)
Description:
The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.3-1ug/ml. Approximately 55kD ban observed using human colon lysates (calculated MW is 55.3kD according to NP_000860.1). A minor band of unknown identity was also consistently observed at 200kD. This band was successfully blocked by incubation with the immunizing peptide.

Peptide ELISA (titer): 1:28,000

Optimal dilutions to be determined by the researcher.

Positive Control: Human Colon lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, CGPQDFEKSPRDR, representing the internal region of the human protein according to NP_998786.1; NP_000860.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Species Crossreactivity: Human. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Dremencov E, Weizmann Y, Kinor N, Gispan-Herman I, Yadid G. Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response. Curr Drug Targets. 2006 Feb;7(2):165-75. Review. PMID: 16475958