Technical Data
S1001-19P
Serotonin Receptor 2A (5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT2A, 5-HT-2A, HTR2, Serotonin receptor 2A)
Description:
5HT are receptors for neurotransmitter and peripheral signal mediator serotonin. It is also known as 5-hydroxytryptamine or 5HT. 5HT receptors mediate the effects of serotonin as the endogenous ligand and of a broad range of pharmaceutical and hallucinogenic drugs. Specifically, 5HT receptors affect the release of other neurotransmitters such as glutamate, dopamine, and GABA as well as their activities. 5HT2A is a type of receptor that belongs to the serotonin receptor family and is one member of G protein coupled receptor (GPCR) family. 5HT2A is involved in the contraction of tracheal smooth muscle, bronchoconstriction, and the control of aldosterone production. The receptor also happens to be necessary for the spread of the human polyoma virus called JC virus. 5HT2A is widely expressed throughout the central nervous system (CNS). It is expressed near most of the serotoninergic terminal rich areas, including neocortex and olfactory tubercle. Increased stimulation of 5HT2A receptors seems to oppose the therapeutic actions of increased stimulation of other serotonin receptors in anti-depressant and anxiolytic treatments.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to the third intracellular domain loop of human Serotonin Receptor 2A, conjugated with KLH. (Swiss Prot: P28223).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Specificity:
Recognizes endogenous levels of human Serotonin Receptor 2A. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.