		Technical Data
S1001-19R	HTR3A (5-hydroxytryptamine Receptor 3A, 5-HT3-A, 5-HT3A, 5-hydroxytryptamine Receptor 3, 5-HT-3, 5-HT3R, Serotonin Receptor 3A, Serotonin-gated Ion Channel Receptor, 5HT3R, HTR3)

Description:
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.

Applications:
Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:128,000
Western Blot: 0.3-1ug/ml, observed in human colon lysates on ~55kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-GPQDFEKSPRDR, from HTR3A, at the internal region of the protein (NP_998786.2, NP_000860.2, NP_001155244.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes HTR3A. Species Crossreactivity: Human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lu CL, Hsieh JC, Dun NJ, Oprea TI, Wang PS, Luo JC, Lin HC, Chang FY, Lee SD. Estrogen rapidly modulates 5-hydroxytrytophan-induced visceral hypersensitivity via GPR30 in rats. Gastroenterology. 2009 Sep;137(3):1040-50.