Technical Data
Serotonin Receptor 7 (5-HT7)
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: >1:100
Immunohistochemistry: 1:500-1:1000 in PBS.
Bn/SA-HRP Technique:
Perfusion Fixation: Fixative-4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20 minutes.
Post Fixation: 4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20 minutes.
Post Fixation: 1.5 hour at 4C. in 4% paraformaldehyde in 0.1M PBS, pH 7.4.
Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.
Incubation on Tissue: 16-24 hours at 4C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat cortex and hippocampus

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceRatRabbit
Not Determined
Synthetic peptide corresponding to aa 8-23 of the rat 5-HT7 receptor
Purified by affinity chromatography.
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O. Dilute further with PBS or Tris buffer.
Recognizes rat Serotonin Receptor 7. Species Crossreactivity: mouse and human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.