Technical Data
Serotonin Receptor 7 (5-HT7)
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100
Immunohistochemistry (Bn-SA/HRP Technique): 1:100-1:300 in PBS.
Immunohistochemistry (Bn/Av-HRP Technique): 1:500-1:1000 in PBS.
Optimal dilutions to be determined by the researcher.

Perfusion Fixation: Fixative-4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20 minutes.
Post Fixation: 4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20min at RT or 1.5hr at 4C. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.
Sections: 50um vibratome
Adsorption control: Synthetic peptide corresponding to aa8-23 of the rat 5-HT7 receptor
Incubation on Tissue: 16-24 hours at 4C.

Positive Control:
Rat cortex and hippocampus tissues

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceRatRabbit
Not Determined
Synthetic peptide corresponding to aa 8-23 of the rat 5-HT7 receptor
Purified by affinity chromatography.
Supplied as a liquid.
Recognizes rat Serotonin Receptor 7. Species Crossreactivity: mouse and human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.