Technical Data
S1001-23N
Serotonin Receptor 7 (5-Hydroxytryptamine (Serotonin) Receptor 7, 5-Hydroxytryptamine Serotonin Receptor 7, 5-HT7, 5-HT-7, 5HT7 Receptor, 5-HT-X, GPRFO, HTR7, Serotonin 5-HT-7 Receptor)
Description:
The neurotransmitter, serotonin, is thought to play a role in various cognitive and behavioral functions. The serotonin receptor encoded by this gene belongs to the superfamily of G protein-coupled receptors and the gene is a candidate locus for involvement in autistic disorder and other neuropsychiatric disorders. Three splice variants have been identified which encode proteins that differ in the length of their carboxy terminal ends

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:16,000
Western Blot: 1-3ug/ml; ~50kD band observed in lysates of cell line KELLY (calculated MW of 49.5kD according to NP_000863.1). An additional band of unknown identity was also consistently observed at 37kD. This band was successfully blocked by incubation with the immunizing peptide.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to QNADYCRKKGHDS, from the C-Terminus of human Serotonin Receptor 7 (NP_000863.1.).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Serotonin Receptor 7 (HTR7). Expected to recognize isoform a (NP_000863.1) only.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Sjögren B, Svenningsson P, Depletion of the lipid raft constituents, sphingomyelin and ganglioside, decreases serotonin binding at human 5-HT7(a) receptors in HeLa cells. Acta physiologica (Oxford, England) 2007 May 190 (1): 47-53.