		Technical Data
S1001-23R	Serotonin Receptor 7 (5-Hydroxytryptamine (Serotonin) Receptor 7, 5-Hydroxytryptamine Serotonin Receptor 7, 5-HT7, 5-HT-7, 5HT7 Receptor, 5-HT-X, GPRFO, HTR7, Serotonin 5-HT-7 Receptor)

Description:
Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Rat	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa13-28 of the rat 5-HT7R (Genbank Accession no. AAA42134.1).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes rat Serotonin 5-HT7 Receptor. Species Crossreactivity: canine, human, mouse. Species sequence homology: porcine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hedlund PB and JC Sutcliffe. 2004. Functional, molecular and pharmacological advances in 5-HT7 receptor research. Trends Pharmacol Sci. 25:481-486. 2. Hoyer D, DE Clarke, JR Fozard, PR Hartig, GR Martin, EJ Mylecharane et al. 1994. International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (serotonin). Pharmacological Reviews 46:157-203.