Technical Data
SHC (Src Homology 2 Domain-containing-transforming Protein C1, SH2 Domain Protein C1, SHC-transforming Protein 1, SHC1, FLJ26504, SHC-transforming Protein 3, SHC-transforming Protein A, SHCA)
The adapter protein Shc was initially identified as an SH2 containing proto-oncogene involved in growth factor signaling. Since then, a number of studies in multiple systems have implicated a role for Shc in many different types of signal transduction including growth factor, antigen, cytokine, G-protein, hormone, and integrin receptor signaling. In addition to the ubiquitous ShcA, there are two other shc gene products, ShcB and ShcC, which are predominantly expressed in neuronal cells. ShcA knockout mice are embryonic lethal and have clearly suggested an important role for ShcA in vivo. An important role for Shc in the activation of MAPK pathway has been established. Thus, Shc adapter proteins are critical components of signal transduction pathways involved in many different cellular processes.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat, HeLa, and SKN-SH cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to aa residues within the C-terminal region of human ShcA.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% gylcerol.
Recognizes human ShcA. Species Crossreactivity: rat, mouse, rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.