Technical Data
S1012-23
SET 7/9
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Technical Data |
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S1012-23 |
SET 7/9 |
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Description: SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with Sadenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET7/9, unlike most other SET proteins, is exclusively a monomethylase. Hybridoma: Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SET7/9. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:2000; Recombinant SET7/9 was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-SET7/9 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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