Technical Data
S1012-23
SET 7/9
Description:
SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with Sadenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET7/9, unlike most other SET proteins, is exclusively a monomethylase.

Applications:
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000; Recombinant SET7/9 was resolved by electrophoresis, transferred to PVDF membrane and probed with S1012-23. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system.
Flow Cytometry: 2-5ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k4k15Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
As Reported
Immunogen:
Recombinant protein corresponding to aa1-366 from human SET 7/9 (NP_085151), purified from E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4 and 0.02% sodium azide, 10% glycerol.
Specificity:
Recognizes human SET 7/9.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bing, X., et al., Nature 421: 652-656 (2003). 2. Taewoo, K., et al., EMBO 22: 292-303 (2003). 3. Nishioka, K., et al., Genes Dev. 16: 479-489 (2002).