		Technical Data
S1012-88D	SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) (Biotin)

Description:
SFRP3, also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt
signaling.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml; The detection limit is ~5ng/lane under non-reducing and reducing conditions. In this format, shows ~60% crossreactivity with recombinant human SFRP3.
Immunohistochemistry: 2-5ug/ml; Detects SFRP3 in cells and tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Mouse	Goat

Concentration:

~0.05mg/ml

Immunogen:

Recombinant corresponding to mouse SFRP3 expressed in NS0 cells.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA.

Specificity:

Recognizes mouse SFRP3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.