Technical Data
S1012-96F
Shh (Sonic HedgeHog)
Description:
Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45kD precursors that undergo auto-cleavage to generate a 19kD amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG8A36Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide (KLH-coupled) corresponding to residues surrounding Glu53 of human Shh.
Purity:
Supernatant
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human Shh protein. Does not cross-react with transfected IHH and DHH. Species Crossreactivity: rat Species Sequence Homology: mouse, Xenopus, zebrafish
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Benameur, T. et al., (2010) PLoS ONE 5: e12688. doi:10.1371/journal.pone.0012688. 1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087. 2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114. 3. Zhang, X.M. et al. (2001) Cell 106, 781-792. 4. Adolphe, C. et al. (2004) Development 131, 5009-5019. 5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117. 6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.