Technical Data
S1012-96J
Shh (C-Product) (Sonic Hedgehog Protein)
Description:
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other members in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatalytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Immunohistochemistry: 1:100
Flow Cytometry: 1:40
Immunoprecipitation: 1:70
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E541Supernatant
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues within c-product subunit of human SHH protein.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris- Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium azide, 0.05% BSA, and 40% glycerol.
Specificity:
Recognizes human Sonic Hedgehog Protein / SHH (C-Product). Specific for both full length (50kD) and c-product subunit (27kD) of human SHH protein. Species crossreactivity: Does not recognize rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Taipale J and Beachy PA. Nature , 411:349354, 2001. 2. Rowbotham NJ, et al. Blood 109(9):3757-3766, 2007. 3. Bhardwaj G, et al. Nat Immunol 2:172180, 2001. 4. Roessler E, et al. Hu Mol Gene, 6:1847-1853.1997.