Technical Data

Media

Shields and Sang M3 Insect Medium w/ L-Glutamine, w/o Glucose (Powder)

S1013-02

Cell Culture Grade

Storage RT/4°C Shipping RT

Components shown as g/L

Inorganic Salts:
Calcium Chloride	0.760
Magnesium Sulfate	2.149
Potassium Bicarbonate	0.500
Sodium Phosphate Monobasic	0.880

Amino Acids:
ß-Alanine	0.250
L-Alanine	1.500
L-Arginine	0.500
L-Asparagine	0.300
L-Aspartic Acid	0.300
L-Cysteine•HCl	0.200
L-Glutamic Acid•K	7.880
L-Glutamic Acid•Na	6.530
L-Glutamine	0.600
Glycine
L-Histidine	0.550
L-Isoleucine	0.250
L-Leucine	0.400
L-Lysine•HCl	0.850
L-Methionine	0.250
L-Phenylalanine	0.250
L-Proline	0.400
L-Serine
L-Threonine	0.500
L-Tryptophan	0.100
L-Tyrosine•2Na	0.360
L-Valine

Vitamins:
Choline Chloride	0.050
Oxalacetic Acid	0.250

Other:
D(+)-Glucose	absent
BIS-TRIS
Yeast Extract	1.000

Total:	29.860g/L

Shields and Sang M3 Insect medium supports the rapid growth of both primary and established cultures of Drosophila cell lines.

Directions per Liter: Dissolve 29.86 grams in 900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume of 1L. Filter-sterilize using a 0.22 micron membrane. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to off white, homogeneous, free flowing powder

Solubility: Colorless to light yellow, clear, complete. Slight precipitate may form.

pH: As reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Shields, G., Sang, J.H.: Improved medium for culture of Drosophila embryonic cells. Drosophila Information Service 52: 161 (1977). 2. Shields, G., Dubendorfer, A., Sang, J.H.: Differentiation in vitro of larval cells from early embryonic cells of Drosophila melangaster. J. Embryol. Exp. Morphol. 33: 159-175 (1975).