Technical Data
Sialyl Lewis x
Sialyl Lewis X, also known as sialyl LeX and SLeX, is a tetrasaccharide carbohydrate that is usually attached to O-glycans on the surface of cells. It is known to play a vital role in cell-to-cell recognition processes. Sialyl Lewis X is one of the most important blood group antigens and is displayed on the terminus of glycolipids that are present on the cell surface. The Sialyl Lewis X determinant, E-selectin ligand carbohydrate structure, is constitutively expressed on granulocytes and monocytes and mediates inflammatory extravasation of these cells. Resting T and B lymphocytes lack its expression and are induced to strongly express sialyl Lewis X upon activation. The Sialyl Lewis X determinant is expressed preferentially on activated Th1 cells but not on Th2 cells.

Suitable for use in Immunohistochemistry, Flow Cytometry, ELISA, Western Blot and Adhesion Blocking. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:40
Flow Cytometry: 10ug/ml
Western Blot: ~1ug/ml
Adhesion Blocking: 10-100ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Control:
Epithelium of bronchus, colon, or pancreatic glands, also adenocarcinomas

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanMouse
Human lung adenocarcinoma cells.
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Recognizes human Sialyl Lewis x epitope expressed on glycoprotein or glycolipid. Reacts strongly with adenocarcinomas and a portion of squamous cell, epithelial, small-cell, large cell, cholangioma, stomach, colon, pancreatic and breast cancers. Also reacts with cervic endometrium, ovary mucinic cystic gland and cystic cancers. In normal adult tissues, reacts with epithelium and bronchus gland of lung, part of colon gland, stomach gland, germ cells, epithelium of pancreatic duct, aciner cells, gall duct epithelium, renal tubule epithelium and part of myeloid cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kenya, S., et al., Cancer Res. 47: 1267-1272 (1987). 2. Oka, T., et al., Oncology & Chemotherapy 3: 363-367 (1987). 3. Kenya, S., et al., Anticancer Res. 9: 999-1004 (1989). 4. Akiko, F., et al., Anticancer Res. 12: 27-32 (1992). 5. Katsutoshi, S., et al., J. Biol. Chem. 268: 22,782-22,787 (1993). 6. Taeko, D., et al., Anticancer Res. 13: 1277-1282 (1993). 7. Katsutoshi, S., et al., J. Biol. Chem. 269: 14,730-14,737 (1994). 8. Kisaburo, N., et al., J. Biol. Chem. 269: 23,290-23,295 (1994)..