Technical Data
S1013-87T3
SIRT1 (SIRT1 Sir2-like Proteins (Sirtuins) Type 1, Sirtuin, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin Type 1, BA57G10.4, hSIRT1, NAD-dependent Deacetylase SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like
Description:
Insulin resistance is an important risk factor in the development of type 2 diabetes and the matabolic syndrome. A new study in Cell Metabolism (1) shows that SIRT1 can ameliorate insulin resistance by silencing expression of protein tyrosine phosphatase 1B.
Sirt1, an HDAC human homologue of the yeast Sir2 protein important in establishing repressive chromatin structures, has been found to associate with the tumor suppressor protein p53. It binds and deacetylates p53 in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. It has also been shpwn to play a role in increasing lifespan.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000-1:10,000
Immunoprecipitation: 1-4ug/mg lysate
Optimal dilutions to be determined by the researcher.

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Peptide mapping to a region between residue 700 and the C-terminus (residue 747) of human Silent Mating Type Information Regulation 2 homolog 1 using the numbering given in entry NP_036370.2 (GeneID 23411).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
Specificity:
Recognizes human SIRT1. Other species have not been tested. Preliminary data using whole cell lysate from NIH3T3 cells failed to provide evidence that S1013-88A7 will bind mouse Sirt1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sun C. et al. SIRT1 Improves Insulin Sensitivity under Insulin-Resistant Conditions by repressing PTP1B. Cell Metabolism 6: 307-319, 2007.