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Technical Data |
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S1013-87T3 |
SIRT1 (SIRT1 Sir2-like Proteins (Sirtuins) Type 1, Sirtuin, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin Type 1, BA57G10.4, hSIRT1, NAD-dependent Deacetylase SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like |
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Description: Insulin resistance is an important risk factor in the development of type 2 diabetes and the matabolic syndrome. A new study in Cell Metabolism (1) shows that SIRT1 can ameliorate insulin resistance by silencing expression of protein tyrosine phosphatase 1B. Sirt1, an HDAC human homologue of the yeast Sir2 protein important in establishing repressive chromatin structures, has been found to associate with the tumor suppressor protein p53. It binds and deacetylates p53 in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. It has also been shpwn to play a role in increasing lifespan. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2000-1:10,000 Immunoprecipitation: 1-4ug/mg lysate Optimal dilutions to be determined by the researcher. Cellular Localization: Nuclear Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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