		Technical Data
S1013-87T5	SIRT1, ID (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1, hSIR2, SIR2L1)

Description:
SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the Center region of human SIRT1 (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human SIRT1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Takata, T., et al., Biochem. Biophys. Res. Commun. 301(1):250-257 (2003). 2.Bitterman, K.J., et al., J. Biol. Chem. 277(47):45099-45107 (2002). 3.Langley, E., et al., EMBO J. 21(10):2383-2396 (2002). 4.Frye, R.A., Biochem. Biophys. Res. Commun. 273(2):793-798 (2000). 5.Frye, R.A., Biochem. Biophys. Res. Commun. 260(1):273-279 (1999).