Technical Data
S1013-88A1
SIRT1 (SIRT1 Sir2-like Proteins (Sirtuins) Type 1, Sirtuin, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin Type 1, BA57G10.4, hSIRT1, NAD-dependent Deacetylase SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like
Description:
Sirt1 (120kD), is a NAD-dependent p53 deacetylase protein involved in diverse processes ranging from regulation of gene silencing to DNA damage response in mammalian cells.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15i88Affinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Ovalbumin-conjugated synthetic peptide corresponding to aa551-599 of human silent information regulator 2 homolog (Sirt1) protein
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, with 40% glycerol
Specificity:
Recognizes human Sirt1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langley, E. et al. (2002) EMBO J. 21, 2383 2396.
2. Vaziri, H. et al. (2001) Cell 107, 149 159.
3. Frye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 279.