Technical Data
S1013-88A1
SIRT1 (SIRT1 Sir2-like Proteins (Sirtuins) Type 1, Sirtuin, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin Type 1, BA57G10.4, hSIRT1, NAD-dependent Deacetylase SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like
Description:
Sirt1 (120kD), is a NAD-dependent p53 deacetylase protein involved in diverse processes ranging from regulation of gene silencing to DNA damage response in mammalian cells.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15i88Affinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Ovalbumin-conjugated synthetic peptide corresponding to aa551-599 of human silent information regulator 2 homolog (Sirt1) protein
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, with 40% glycerol
Specificity:
Recognizes human Sirt1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langley, E. et al. (2002) EMBO J. 21, 2383 – 2396.
2. Vaziri, H. et al. (2001) Cell 107, 149 – 159.
3. Frye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 – 279.