		Technical Data
S1013-88A9	SIRT1 (Sirtuin 1, Sirtuin-1, Sirtuin Type 1, hSIRT1, NAD-dependent Deacetylase Sirtuin-1, hSIR2, SIR2-like Protein 1, SIR2L1)

Description:
The SIRT family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. Sirtuins utilize NAD+ as a substrate to catalyze deacetylation of specific acetylated protein and histones. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR. SIRT1 also regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	7B7	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to aa456-555 from human SIRT1 with GST tag. MW of the GST tag alone is 26kD.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

Recognizes human SIRT1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.