Technical Data
SIRT 3 (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:100
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not Determined
Synthetic peptide derived from the sequence of human SIRT 3 protein (KLH).
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of human SIRT 3 protein, both the uncleaved 45kD precursor protein and the cleaved 28kD protein. Does not crossreact with other Sirtuin proteins. Species Crossreactivity: rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Guarente, L. (1999) Nat Genet 23, 2815. 2. Scher, M.B. et al. (2007) Genes Dev 21, 9208. 3. Schwer, B. et al. (2002) J Cell Biol 158, 64757. 4. Onyango, P. et al. (2002) Proc Natl Acad Sci USA 99, 136538. 5. Schwer, B. et al. (2006) Proc Natl Acad Sci USA 103, 102249. 6. Shi, T. et al. (2005) J Biol Chem 280, 135607. 7. Ashraf, N. et al. (2006) Br J Cancer 95, 105661.