Technical Data
S1013-88D4
SIRT 2 (Sirtuin 2, Sirtuin-2, Sirtuin Type 2, SIRT2, Sir2, NAD-dependent Deacetylase Sirtuin 2, Silencing Information Regulator 2-like, Silent Mating Type Information Regulation 2, Silent Mating Type Information Regulation 2 Homolog, SIR2-like, SIR2L, SIR
Description:
Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2alpha) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein,

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Immunohistochemistry (paraffin): 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9i207Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Sir2. The antibody does not cross-react with other sirtuin family members.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.