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Technical Data |
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S1013-88D4 |
SIRT 2 (Sirtuin 2, Sirtuin-2, Sirtuin Type 2, SIRT2, Sir2, NAD-dependent Deacetylase Sirtuin 2, Silencing Information Regulator 2-like, Silent Mating Type Information Regulation 2, Silent Mating Type Information Regulation 2 Homolog, SIR2-like, SIR2L, SIR |
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Description: Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2alpha) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein, Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Immunohistochemistry (paraffin): 1:100-250 Immunocytochemistry: 1:100-250 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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