Technical Data
SIRT 2 (Sirtuin 2, Sirtuin-2, Sirtuin Type 2, SIRT2, Sir2, NAD-dependent Deacetylase Sirtuin 2, Silencing Information Regulator 2-like, Silent Mating Type Information Regulation 2, Silent Mating Type Information Regulation 2 Homolog, SIR2-like, SIR2L, SIR
Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2alpha) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein,

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Immunohistochemistry (paraffin): 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2.
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human Sir2. The antibody does not cross-react with other sirtuin family members.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.