Description:
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2b | 5i87 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ug | | 4°C (-20°C Glycerol) | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~0.25mg/ml |
| Immunogen: |
| ovalbumin-conjugated synthetic peptide corresponding to aa28 - aa77 of human silent information regulator 2 homolog (Sirt3) |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 40% glycerol. |
| Specificity: |
| Recognizes Sirt3, a mitochondrial NAD-dependent deacetylase, enzyme involved in the constitutive deacetylation of one or several mitochondrial proteins. The Sirtuin 3 activity could play an important role in the mechanism of the acetylation control of factors directly involved in apoptotic pathways. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Schwer, B. et. al. (2002) J. Cell Biol. 158, 647 - 657.
2. Smith, J.S. et. al. (2002) Methods Enzymol. 353, 282 - 300.
3. Frye, R. (2000) Biochem. Biophys. Res. Commun. 273, 793 - 798.
4. Frye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 - 279.
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