Technical Data
S1013-88F1
SIRT 3 (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b5i87Affinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.25mg/ml
Immunogen:
ovalbumin-conjugated synthetic peptide corresponding to aa28 - aa77 of human silent information regulator 2 homolog (Sirt3)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Specificity:
Recognizes Sirt3, a mitochondrial NAD-dependent deacetylase, enzyme involved in the constitutive deacetylation of one or several mitochondrial proteins. The Sirtuin 3 activity could play an important role in the mechanism of the acetylation control of factors directly involved in apoptotic pathways.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schwer, B. et. al. (2002) J. Cell Biol. 158, 647 - 657.
2. Smith, J.S. et. al. (2002) Methods Enzymol. 353, 282 - 300.
3. Frye, R. (2000) Biochem. Biophys. Res. Commun. 273, 793 - 798.
4. Frye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 - 279.