Technical Data
S1013-88F3
SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae), Sirtuin Type 3, HGNC:14931, hSIRT3, Mitochondrial Nicotinamide Adenine Dinucleotide-dependent Deacetylase, NAD-dependent Deacetylase Sirtuin 3 Mito
Description:
This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (peptide): 1:28,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synethtic peptide: C-QRETGKLDGPDK, representing the C Terminus of the human protein according to NP_036371.1, NP_001017524.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH7.3, containing 0.5% BSA
Specificity:
Recognizes human SIRT3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes.J Biol Chem. 2005 Jan 14; [Epub ahead of print] PMID: 15653680