Technical Data
SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae), Sirtuin Type 3, HGNC:14931, hSIRT3, Mitochondrial Nicotinamide Adenine Dinucleotide-dependent Deacetylase, NAD-dependent Deacetylase Sirtuin 3 Mito
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:128,000
Western Blot: 0.1-0.3ug/ml ~28kD band observed in Human Liver lysates (calculated MW of 28.6kD according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35kD and 48kD are observed. These bands are not observed in the non-transfected HEK293.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-QRETGKLDGPDK, from the C Terminus of human SIRT3(NP_036371.1, NP_001017524.1).
Purified by peptide affinity chromatography.
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Recognizes human SIRT3. This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem. 2005 Jan 14; [Epub ahead of print] PMID: 15653680Auto-generated 26/08/2010