Technical Data
S1013-88F5
SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae), Sirtuin Type 3, HGNC:14931, hSIRT3, Mitochondrial Nicotinamide Adenine Dinucleotide-dependent Deacetylase, NAD-dependent Deacetylase Sirtuin 3 Mito
Description:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:128,000
Western Blot: 0.1-0.3ug/ml ~28kD band observed in Human Liver lysates (calculated MW of 28.6kD according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35kD and 48kD are observed. These bands are not observed in the non-transfected HEK293.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QRETGKLDGPDK, from the C Terminus of human SIRT3(NP_036371.1, NP_001017524.1).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human SIRT3. This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem. 2005 Jan 14; [Epub ahead of print] PMID: 15653680Auto-generated 26/08/2010