Technical Data
S1013-88F7
SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Type 3, HGNC:14931, hSIRT3, NAD-dependent Deacetylase Sirtuin-3 Mitochondrial, Sir2-like 3, SIR2 Like Protein 3, SIR2-like Protein 3, SIR2L3)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
ELISA: >1:128,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Liver lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QRETGKLDGPDK, from the C-terminus of the protein sequence according to NP_036371.1, NP_001017524.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human SIRT3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem. 2005 Jan 14; [Epub ahead of print] PMID: 15653680