		Technical Data
S1013-88F7	SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Type 3, HGNC:14931, hSIRT3, NAD-dependent Deacetylase Sirtuin-3 Mitochondrial, Sir2-like 3, SIR2 Like Protein 3, SIR2-like Protein 3, SIR2L3)

Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
ELISA: >1:128,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Liver lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-QRETGKLDGPDK, from the C-terminus of the protein sequence according to NP_036371.1, NP_001017524.1.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes human SIRT3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem. 2005 Jan 14; [Epub ahead of print] PMID: 15653680