Technical Data
SIRT1 (SIRT1 Sir2-like Proteins (Sirtuins) Type 1, Sirtuin, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin Type 1, BA57G10.4, hSIRT1, NAD-dependent Deacetylase SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like
Telomeric DNA is bound by the transcription regulator Rap1 (repressor activator protein 1). Rap1 is involved in the repression of genes located adjacent to the telomeres, known as telomere position effect (TPE) (1). The silent information regulator proteins Sir2, Sir3, and Sir4 form a complex with Rap1 (2,3). Sir1 is essential for silencing the HM mating-type loci, but has no effect on TPE (4).

Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:5,000 (82-110kD)
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:50

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul -20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues in C-terminus of human Sir2/SIRT1. was used as immunogen.
Supplied as a liquid.
Recognizes human Sir2/SIRT1. Does not cross-react with other Sirtuin family members.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kyrion, G., et al. RAP1 and telomere structure regulate telomere position effects in Saccharomyces cerevisiae. Genes and Dev. 7: 1146–1159 (1993).
2. Palladino, F., et al. SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres. Cell 75: 543–555 (1993).
3. Cockell, M., et al. The carboxy termini of Sir4 and Rap1 affect Sir3 localization: evidence for a multicomponent complex required for yeast telomeric silencing. J. Cell Biol. 129: 909–924 (1995).
4. Tsukamoto, Y., et al. Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiae. Nature 388: 900–903 (1997).