Technical Data
S1013-88G2
SIRT1, phosphorylated (Ser27) (SIRT1 Sir2-like Proteins (Sirtuins) Type 1, Sirtuin, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin Type 1, BA57G10.4, hSIRT1, NAD-dependent Deacetylase SIRT1, NAD-dependent Deacetyl
Description:
The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2, SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Applications:
Suitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:10,000
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9E548Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A phospho-specific peptide corresponding to residues surrounding serine 27 of human Sir2/SIRT1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human Sir2/SIRT1 phosphorylated on serine 27.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. David A. Sinclair et al; J. Biol. Chem., Vol. 277, Issue 47, 45099-45107, November 22, 2002 2. Smith, J. Set al. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 6658-6663 3. Kouzarides et al, EMBO Journal, Vol. 21, No. 10 pp. 2383- 2396, 2002 4. Tanner, K. G.et al (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 14178-1418288: 900–903 (1997).