Technical Data
S1013-88H
SIRT4 (Sirtuin 4, Sirtuin-4, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 4 (S. cerevisiae), Sirtuin Type 4, NAD-dependent Deacetylase Sirtuin 4, Silent Mating Type Information Regulation 2 Homolog, Sir2 Like 4, Sir2-like 4, SIR2 Like Pro
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15i86Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
ovalbumin-conjugated synthetic peptide corresponding to N-terminal aa1-50 of human silent information regulator 2 homolog (Sirt4)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes Sirt4, a NAD-dependent deacetylase, protein involved in diverse processes ranging from regulation of gene silencing to DNA damage response in mammalian cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smith, J.S. et. al. (2002) Methods Enzymol. 353, 282 - 300.
2. Frye, R. (2000) Biochem. Biophys. Res. Commun. 273, 793 - 798.
3. Frye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 - 279.