Technical Data
SIRT 4 (Sirtuin 4, Sirtuin-4, Sirtuin Type 4, MGC57437, MGC130046, MGC130047, NAD-dependent ADP-ribosyltransferase Sirtuin-4, Sir2 Like 4, Sir2-like 4, SIR2 Like Protein 4, SIR2-like Protein 4, SIR2L4)
SIRT4, otherwise known as sirtuin 4, is a mitochondrial NAD-dependent ADP-ribosyl transferase, and member of the sirtuin family, which catalyzes the transfer of ADP-ribosyl groups onto target proteins. Studies have shown that SIRT4 acts as a repressor of GDH (glutamate dehydrogenase) activity in beta cell mitochondria, thereby suppressing amino-acid dependent insulin production.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Kidney lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to SRCGELLPLIDPC, from the C-terminus of the protein sequence according to NP_036372.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Recognizes human SIRT4. Species sequence homology: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Frye RA. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.Biochem Biophys Res Commun. 1999 Jun 24;260(1):273-9.PMID: 10381378