Technical Data
S1013-88J1
SIRT 5 (Sirtuin 5, Sirtuin-5, Sirtuin Type 5, NAD-dependent Deacetylase Sirtuin 5, Silent Mating Type Information Regulation 2 (S. cerevisiae) Homolog 5, Sir2-like 5, SIR2L5)
Description:
SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15i85Affinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Ovalbumin-conjugated synthetic peptide corresponding to N-terminal aa1-50 of human silent information regulator 2 homolog (Sirt5)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Specificity:
Recognizes human Sirt5, a NAD-dependent deacetylase, protein involved in diverse processes ranging from regulation of gene silencing to DNA damage response in mammalian cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Smith, J.S. et. al. (2002) Methods Enzymol. 353, 282 - 300.