Technical Data
S1013-88K
SIRT6, CT (Sirtuin 6, Sirtuin-6, Mono-ADP-ribosyltransferase Sirtuin-6, SIR2-like Protein 6, SIR2L6)
Description:
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging. SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway. The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2. In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres and gaps. SirT6 may regulate the BER pathway by deacetylating DNA Pol-beta or other core components of the pathway.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence: (IC) 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of the human SirT6 protein.
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Detects endogenous levels of human total SirT6 protein at 42kD. This antibody does not cross-react with other sirtuin proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Guarente, L. (1999) Nat Genet 23:2815. 2. Mostoslavsky, R. et al. (2006) Cell 124:31529. 3. Liszt, G. et al. (2005) J Biol Chem 280:2131320. 4. Michishita, E. et al. (2005) Mol Biol Cell 16:462335.