Technical Data
S1013-88K1
SIRT6 (Sirtuin 6, Sirtuin-6, Mono-ADP-ribosyltransferase Sirtuin-6, SIR2-like Protein 6, SIR2L6)
Description:
Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6), a mouse Sir2 homolog, is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in the ageing process.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control: MEF lysate

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide made to a C-terminal region of the mouse SIRT6 protein (within residues 250-334). [Swiss-Prot P59941].
Purity:
Purified by immunoaffinity chromatography.
Form
Tris-citrate/phosphate (pH 7-8)
Specificity:
Species Crossreactivity: Mouse. Other species have not been tested. The immunogen used for the production of this antibody shows 92% homology with the rat protein and 78% with the human protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liszt, G., et al. Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase. JBC. 280(22): 21313-21320 (2005)