Technical Data
SIRT 7 (Sirtuin 7, Sir2-like Protein Type 7)
SIRT7 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human liver or PBMC

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Synthetic peptides corresponding to amino acids 35-51 and 361-377 of human SIRT7.
Purified by Protein G chromatography
Supplied as a liquid in PBS, 0.02% sodium azide, 0.1 mg/ml BSA.
Recognizes human SIRT 7 (Sirtuin 7)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Frye RA. Biochem Biophys Res Commun 260: 273-279 (1999).
2. Landry J, Sutton A, Tafrov ST. Heller RC, Stebbins J, et al. Proc Natl Acad Sci USA 97: 5807-5811 (2000).
3. Imai S, Armstrong CM, Kaeberlein M, Guarente L. Nature 403: 795-800 (2000).
4. Frye RA. Biochem Biophys Res Commun 273: 793-798 (2000).